Prevalence, antifungal susceptibility and virulence determinants of oral yeast species isolated from immunodeficient patients in Northeastern Brazil

Autores

• João Pedro Cavalcante UFAL
• Gustavo Vasconcelos Bastos Paulino Universidade Federal de Alagoas, Maceió, AL, Brasil. https://orcid.org/0000-0003-2825-3133
• Leonardo Broetto Universidade Federal de Alagoas, Campus Arapiraca, AL, Brasil. https://orcid.org/0000-0002-5424-2115
• Thayná Melo de Lima Morais Universidade de Campinas - UNICAMP
• Sonia Maria Soares Ferreira Centro de Estudos Superiores de Maceió - CESMAC https://orcid.org/0000-0002-4825-171X
• Patricia Valente Universidade Federal do Rio Grande do Sul - UFRGS https://orcid.org/0000-0002-4825-171X
• Melissa Fontes Landell Universidade Federal de Alagoas, Maceió, AL, Brasil. https://orcid.org/0000-0001-6848-0803

DOI:

https://doi.org/10.17058/jeic.v10i3.15134

Palavras-chave:

Virulence Factors. Oral Candidiasis. Acquired Immunodeficiency Syndrome. Yeasts. Candida.

Resumo

Background and Objectives: Oral candidiasis has a common occurrence in immunocompromised patients. However, other emergent infections have become increasingly common. The aim of this study was to investigate the prevalence, virulence determinants and the antifungal susceptibility of yeast colonizing the mucosa of immunocompromised patients in Northeastern Brazil. Methods: Samples from sixty HIV-positive patients seen at the Specialized Service / Hospital Dia - Hospital Universitário Prof. Alberto Antunes from the Federal University of Alagoas were collected from subgingival sites and seeded on CHROMagar for presumptive confirmation of Candida spp. followed by PCR and sequencing. In addition, we tested virulence determinants, phospholipase and protease and evaluated in vitro the Minimum Inhibitory Concentration of antifungals amphotericin B and fluconazole. This project was approved by the Research Ethics Committee of the Center for Higher Studies in Maceió. Results: Approximately 63% of the patients were colonized by yeasts, with C. albicans as the predominant species, while non-Candida albicans species accounted for 49% of the isolates, with C. dubliniensis and C. parapsilosis being the commonest, but C. intermedia, Bullera penniseticola and Naganishia liquefaciens were also found. The virulence determinants protease and/or phospholipase were also produced by Candida spp. and some uncommon opportunistic isolates such as Kodamaea ohmeri, N. liquefaciens and Saitozyma podzolica. Furthermore, most of Candida spp. strains and some uncommon opportunistic species showed high values of minimal inhibitory concentration. Conclusion: Results obtained indicate that C. albicans continues to be the predominant species in oral cavity of immunodeficient patients and along with other unusual species may present high resistance to the antifungals tested.